QC techniques (electrophoresis, spectrophotometer)

In the determination of the fluorescent-based concentration, nucleic acids or proteins labeled with fluorescent dyes are used to accurately determine the concentration of samples. Fluorescence detection provides a high sensitivity for quantifying molecules, allowing precise measurement of small amounts of samples. In combination with spectrophotometric measurements, this technique is particularly useful for determining the amount of nucleic acids and proteins quickly and reliably.

Size-based separation performs separation based on the size and electric charge of the molecules. Nucleic acids and proteins migrate in the gel at different rates, which allows for the identification and analysis of different fragments of samples. This technique is fundamental in molecular biology studies, such as gene fragmentation or protein profiling.

Optical density (OD) measurement is used to determine the concentration of a substance using spectrophotometry, especially for nucleic acids and proteins. Based on the OD measurement, the absorbance of the sample (which indicates the absorption of light) can be determined and related to the concentration of the molecule. This technique is applied to quality control to ensure the purity and integrity of the sample.